An examination of leaf beetle specimens (Coleoptera: Chrysomelidae) in the five largest beetle collections in Kentucky, recent inventory work in state nature preserves, and a review of the literature revealed four species of the subfamily Donaciinae and eleven species of Criocerinae present in Kentucky, six of which are new state records. Distribution maps and label data are presented for fifteen Kentucky species of the subfamilies Donaciinae and Criocerinae, including spatial (state and Kentucky county records), temporal (years and months of collection in Kentucky), and plant association information. The following species are reported from Kentucky for the first time: Donacia limonia Schaeffer, Crioceris duodecimpunctata (Linnaeus), Neolema cordata White, N. ovalis White, Oulema collaris (Say), and O. sayi (Crotch).